Research interests

  • Post-testicular maturation of spermatozoa
  • Oxidative stress and sperm quality
  • Epignenetic of male gametes

The research interests of the MePTI team (Mechanisms of Post-Testicular Infertility) are centered on male gametes maturation in the epididymis, and dysregulations that may lead to infertility. Using our genetically modified mouse models, we address several biological questions:

  • What is the impact of epididymal oxidative stress on the genetic and epigenetic materials of male gametes?
  • How does a dyslipidemia modify the epididymis physiology, the composition, the structure and the function of male gametes?
  • How does the epididymis answer to the immune challenge represented by a particular dual situation: avoid an autoimmune response against male gametes and in the same time protect the organ and the gametes against ascending pathogens?

These topics are investigated using different techniques and technologies: classical male gametes biology (motility, morphology, vitality, acrosome reaction, DNA fragmentation by a semi-assisted analysis platform), DNA oxidation status evaluated by immunofluorescence and confocal microscopy, oxidized-DNA immunoprecipitation (Oxidip) and new generation sequencing, flow cytometry and cell sorting, molecular biology (qRT-PCR, western-blot…).

Members of the laboratory

Joël Drevet, PhD
University professor, team leader

Joëlle Henry-Berger, PhD
Research Engineer

Rachel Guiton, PhD
Assistant Professor

Stéphanie Bravard
 Technical assistant

Chantal Goubely, MSc
Study Engineer

Christelle Damon, BSc
Assistant Engineer

Areski Chorfa, BSc
Study Engineer