The objective of this platform is to assist RQR members in the creation of genome-edited animals for research. Genome editing enables the study of gene functions, the modulation of gene sequences controlling phenotypic traits (e.g., improve welfare, health and product quality), and the production of animal models to study pathophysiology mechanisms. We offer extensive expertise in the production of genome-edited animals and several assisted reproductive technologies.
Services offered:
- Large animal genome editing
- Design and validation of gene editing tools in zygotes/embryos and cells
- Knockout/Knock-in embryos and cells
- In vitro and in vivo embryo production
- Cloning by somatic cell nuclear transfer (SCNT)
- Intra-cytoplasmic sperm injection (ICSI)
- Laparoscopic ovum pick-up (domestic and wild animal species)
- Prepubertal reproductive technologies
The RQR laboratory animal genome editing platform allows its members to create genetically modified animals. It provides researchers with the support required to establish and maintain their animal colonies and can be consulted for any questions regarding colonies of genetically modified mice or rats.
Turnkey or à la carte Crispr/Cas9 gene editing services:
- Gene editing and genotyping design strategy
- Guide RNA selection and oligo donor design
- Guide screening/testing in embryos
- Microinjection
- Founder animal genotyping
Efficient protocols and proven track record for the generation of:
- Indel and exon deletion
- Insertion of point mutations and short sequence tags
- Insertion of loxP site for the generation of conditional alleles
- Large Knock-ins
Other services:
- Crispr/Cas9 rodent model generation
- Microinjection of ES cells
- Production of gene-targeted mice
- Murine embryonic stem cell targeting
- Embryo rederivation
- Cryopreservation of embryos and sperm
- Assisted reproduction technologies
